Shah! Viruses can hear – and see!

br>

bacteriophage artist's concept

Viruses are utilizing info from their surroundings to “resolve” when to sit down inside their hosts and when to multiply and burst, killing the host cell, in line with a brand new scientific examine. This illustration reveals a bacteriophage.

New analysis reveals viruses can have “eyes and ears” on us

The newly found, widespread potential of some viruses to watch their surroundings could have implications for antiviral drug improvement.

New analysis signifies that viruses are utilizing info from their surroundings to “resolve” when to sit down tightly inside their host and when to multiply and burst, killing the host cell. The work has vital implications for antiviral drug improvement. Led by the College of Maryland Baltimore County (UMBC), the examine was just lately printed Frontiers in Microbiology,

,[I]Whereas F phages are listening on their hosts, viruses affecting people are doing the identical.” – ivan arily

A virus’s potential to sense its surroundings, together with components produced by its host, provides “one other layer of complexity to viral-host interactions,” says Ivan Aril. He’s senior creator on the brand new paper and professor of organic sciences at UMBC. Proper now the virus is benefiting from that potential and taking its advantages. However he says that sooner or later, “we could exploit this to their detriment.”

no coincidence

The brand new examine centered on bacteriophages, also known as “phages.” They’re viruses that infect micro organism. Within the examine, the phages analyzed can solely infect their hosts when bacterial cells have specialised appendages, known as pili and flagella, that assist micro organism transfer and mate. The micro organism produce a protein known as CtrA that controls the manufacturing of those appendages. Analysis has proven that many appendage-dependent phases have patterns in them.[{” attribute=””>DNA where the CtrA protein can attach, called binding sites. Erill says that a phage having a binding site for a protein produced by its host is unusual.

Delta Phage

A delta bacteriophage, the first identified in a new study in Frontiers in Microbiology to have binding sites for CtrA, a protein produced by the bacteriophage’s host that regulates the production of pili and flagella. The presence of these binding sites only in phages that require their host cells to have pili/flagella in order to infect them suggests that the phage is monitoring the presence of this protein in order to “decide” whether to stay put or replicate and emerge from its host cell. Credit: Tagide deCarvalho/UMBC

Even more surprising, Erill and the paper’s first author Elia Mascolo, a Ph.D. student in Erill’s lab, discovered through detailed genomic analysis that these binding sites were not unique to a single phage, or even a single group of phages. Many different types of phages had CtrA binding sites—but they all required their hosts to have pili and/or flagella to infect them. They decided that it couldn’t be a coincidence.

The ability to monitor CtrA levels “has been invented multiple times throughout evolution by different phages that infect different bacteria,” Erill says. When distantly related species exhibit a similar trait, it’s called convergent evolution—and it indicates that the trait is definitely useful.

Timing is everything

Another wrinkle in the story: The first phage in which the scientists identified CtrA binding sites infects a particular group of bacteria called Caulobacterales. Caulobacterales are an especially well-studied group of bacteria, because they exist in two forms: a “swarmer” form that swims around freely, and a “stalked” form that attaches to a surface. The swarmers have pili/flagella, and the stalks do not. In these bacteria, CtrA also regulates the cell cycle, determining whether a cell will divide evenly into two more of the same cell type, or divide asymmetrically to produce one swarmer and one stalk cell.

Ivan Erill

Ivan Erill. Credit: Marlayna Demond ’11/UMBC

Since the phages can only infect swarmer cells, it’s in their best interest only to burst out of their host when there are many swarmer cells available to infect. Generally, Caulobacterales live in nutrient-poor environments, and they are very spread out. “But when they find a good pocket of microhabitat, they become stalked cells and proliferate,” Erill says, eventually producing large quantities of swarmer cells.

So, “We hypothesize the phages are monitoring CtrA levels, which go up and down during the life cycle of the cells, to figure out when the swarmer cell is becoming a stalk cell and becoming a factory of swarmers,” Erill says, “and at that point, they burst the cell, because there are going to be many swarmers nearby to infect.”

Listening in

Unfortunately, the method to prove this hypothesis is extremely difficult and labor-intensive, so that wasn’t part of this latest paper—although Erill and colleagues hope to tackle that question in the future. However, the research team sees no other plausible explanation for the proliferation of CtrA binding sites on so many different phages, all of which require pili/flagella to infect their hosts. Even more interesting, they note, are the implications for viruses that infect other organisms—even humans.

“If you are developing an antiviral drug, and you know the virus is listening in on a particular signal, then maybe you can fool the virus.” — Ivan Erill

“Everything that we know about phages, every single evolutionary strategy they have developed, has been shown to translate to viruses that infect plants and animals,” he says. “It’s almost a given. So if phages are listening in on their hosts, the viruses that affect humans are bound to be doing the same.”

There are a few other documented examples of phages monitoring their environment in interesting ways, but none include so many different phages employing the same strategy against so many bacterial hosts.

This new research is the “first broad scope demonstration that phages are listening in on what’s going on in the cell, in this case, in terms of cell development,” Erill says. But more examples are on the way, he predicts. Already, members of his lab have started looking for receptors for other bacterial regulatory molecules in phages, he says—and they’re finding them.

New therapeutic avenues

The key takeaway from this research is that “the virus is using cellular intel to make decisions,” Erill says, “and if it’s happening in bacteria, it’s almost certainly happening in plants and animals, because if it’s an evolutionary strategy that makes sense, evolution will discover it and exploit it.”

For example, an animal virus might want to know what kind of tissue it is in, or how robust the host’s immune response is to its infection in order to optimize its strategy for survival and replication. While it might be disturbing to think about all the information viruses could gather and possibly use to make us sicker, these discoveries also open up opportunities for new therapies.

“If you are developing an antiviral drug, and you know the virus is listening in on a particular signal, then maybe you can fool the virus,” Erill says. That’s several steps away, however. For now, “We are just starting to realize how actively viruses have eyes on us—how they are monitoring what’s going on around them and making decisions based on that,” Erill says. “It’s fascinating.”

For more on this research, see Viruses May Be “Watching” You.

Reference: “The transcriptional regulator CtrA controls gene expression in Alphaproteobacteria phages: Evidence for a lytic deferment pathway” by Elia Mascolo, Satish Adhikari, Steven M. Caruso, Tagide deCarvalho, Anna Folch Salvador, Joan Serra-Sagristà, Ry Young, Ivan Erill and Patrick D. Curtis, 19 August 2022, Frontiers in Microbiology.
DOI: 10.3389/fmicb.2022.918015

Funding: National Science Foundation, NIH/National Institutes of Health



Supply hyperlink

Leave a Comment